2V4N
Crystal structure of Salmonella typhimurium SurE at 1.7 angstrom resolution in orthorhombic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 73.730, 121.638, 143.256 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.610 - 1.700 |
| R-factor | 0.18446 |
| Rwork | 0.183 |
| R-free | 0.21227 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j9j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.072 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.480 |
| Number of reflections | 35384 | |
| <I/σ(I)> | 26.3 | 2.33 |
| Completeness [%] | 99.7 | 97.8 |
| Redundancy | 5.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 0.2M TRISODIUM CITRATE DIHYDRATE, 0.1M HEPES PH 7.5, 30% MPD MICROBATCH METHOD |
