2V4J
THE CRYSTAL STRUCTURE OF Desulfovibrio vulgaris DISSIMILATORY SULFITE REDUCTASE BOUND TO DsrC PROVIDES NOVEL INSIGHTS INTO THE MECHANISM OF SULFATE RESPIRATION
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2007-02-24 |
Detector | ADSC CCD |
Wavelength(s) | 1.033, 1.742 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.410, 118.900, 132.240 |
Unit cell angles | 90.00, 104.13, 90.00 |
Refinement procedure
Resolution | 128.040 - 2.100 |
R-factor | 0.1916 |
Rwork | 0.190 |
R-free | 0.21880 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.290 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.050 | 0.490 |
Number of reflections | 112244 | |
<I/σ(I)> | 13.2 | 2 |
Completeness [%] | 98.1 | 88.7 |
Redundancy | 3.1 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 12.5% PEG 4000, 0.1M TRIS-HCL PH 8.5, 0.2M MGCL2 |