2V4E

A non-cytotoxic DsRed variant for whole-cell labeling

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-15
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	115.526, 122.619, 164.851
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.223
Rwork	0.220
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vae
RMSD bond length	0.011
RMSD bond angle	1.434
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.140	0.610
Number of reflections	92897
<I/σ(I)>	14.3	2.2
Completeness [%]	98.0	84.8
Redundancy	6.6	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	100 MM BIS-TRIS, PH 5.5 200 MM AMMONIUM SULFATE, 16% PEG8000, VAPOR DIFFUSION, SITTING DROP, 298K