2V4A
Crystal structure of the SeMet-labeled prolyl-4 hydroxylase (P4H) type I from green algae Chlamydomonas reinhardtii.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9421, 0.9421, 0.9798 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 137.490, 137.490, 88.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.870 - 1.930 |
R-factor | 0.192 |
Rwork | 0.188 |
R-free | 0.22300 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.017 |
RMSD bond angle | 1.526 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | REFMAC (5.3.0028) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.050 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.060 | 0.340 |
Number of reflections | 457239 | |
<I/σ(I)> | 11.8 | 3.8 |
Completeness [%] | 99.1 | 98.1 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |