2V43
Crystal structure of RseB: a sensor for periplasmic stress response in E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-07 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 98.600, 200.700, 109.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.370 |
R-factor | 0.231 |
Rwork | 0.228 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | LARGE DOMAIN FROM P4212 DATASET |
RMSD bond length | 0.011 |
RMSD bond angle | 1.697 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.370 |
Rmerge | 0.110 | 0.580 |
Number of reflections | 40893 | |
<I/σ(I)> | 12.6 | 2.4 |
Completeness [%] | 95.2 | 89.4 |
Redundancy | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | PROTEIN WAS CRYSTALLIZED FROM 0.2 M MAGNESIUM CHLORIDE HEXAHYDRATE, 0.1 M TRIS-HCL PH 8.5, 25%(W/V) POLYETHYLENE GLYCOL 3350 AND 10 MM L-CYSTEINE |