2V42
Crystal structure of RseB: a sensor for periplasmic stress response in E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 164.290, 164.290, 81.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.750 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.25800 |
| Structure solution method | MIR |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.412 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.900 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.090 | 0.510 |
| Number of reflections | 28941 | |
| <I/σ(I)> | 18.8 | 2.4 |
| Completeness [%] | 97.5 | 92.8 |
| Redundancy | 12.4 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PROTEIN WAS CRYSTALLIZED FROM 2.4 M SODIUM MALONATE PH 7, 0.3 M DIMETHYLETHYLAMMONIUM PROPANE SULFONATE |
