2V3T
Structure of the ligand-binding core of the ionotropic glutamate receptor-like GluRdelta2 in the apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-26 |
Detector | MARRESEARCH |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 70.971, 91.031, 177.393 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.750 |
R-factor | 0.2314 |
Rwork | 0.231 |
R-free | 0.28670 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.437 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.000 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.100 | 0.500 |
Number of reflections | 27717 | |
<I/σ(I)> | 20 | 2.2 |
Completeness [%] | 97.6 | 83.2 |
Redundancy | 9.2 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 11% PEG4000, 0.1 M TRIS-HCL PH 8.5, 50 MM CACL2. HANGING DROP VAPOR DIFFUSION. |