2V3M
Structure of the Gar1 domain of NAf1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 103.534, 103.534, 109.031 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.570 - 2.740 |
| R-factor | 0.259 |
| Rwork | 0.257 |
| R-free | 0.28700 |
| Structure solution method | SAD |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.634 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.890 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.090 | 0.590 |
| Number of reflections | 18030 | |
| <I/σ(I)> | 18.9 | 3.3 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 8.9 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 2M AMMONIUM SULPHATE, 0.2M K/NA TARTRATE, NA CITRATE PH5.6., pH 5.60 |
