2V3C

Crystal structure of the SRP54-SRP19-7S.S SRP RNA complex of M. jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	70.286, 129.402, 163.417
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.247
Rwork	0.244
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lng
RMSD bond length	0.021
RMSD bond angle	2.953
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.130	0.760
Number of reflections	49835
<I/σ(I)>	6.3	1.6
Completeness [%]	99.1	95.2
Redundancy	5.6	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1