2V3B
Crystal structure of the electron transfer complex rubredoxin - rubredoxin reductase from Pseudomonas aeruginosa.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-05 |
Detector | MARRESEARCH |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 61.070, 97.150, 81.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 97.130 - 2.450 |
R-factor | 0.185 |
Rwork | 0.181 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2V3A AND 1BQ8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.183 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.100 | 0.400 |
Number of reflections | 19054 | |
<I/σ(I)> | 12.1 | 3 |
Completeness [%] | 90.6 | 86.2 |
Redundancy | 3.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2M KF, 20% PEG 3350 |