2V2U
Structure of Mouse gammaC-crystallin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.911, 46.317, 53.317 |
| Unit cell angles | 108.53, 94.08, 99.37 |
Refinement procedure
| Resolution | 43.070 - 1.900 |
| R-factor | 0.209 |
| Rwork | 0.205 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hk0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.511 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.100 | 2.120 |
| High resolution limit [Å] | 1.900 | 1.930 |
| Rmerge | 0.100 | 0.620 |
| Number of reflections | 28779 | |
| <I/σ(I)> | 4.2 | 0.8 |
| Completeness [%] | 93.5 | 89.1 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M SODIUM ACETATE, 0.1 M SODIUM CACODYLATE PH 6.5 AND 30% PEG 8000 |
