2V2T
X-ray structure of a NF-kB p50-RelB-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 91.440, 91.440, 419.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 3.050 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vkx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.150 |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.060 | 0.510 |
| Number of reflections | 19931 | |
| <I/σ(I)> | 12 | 2.5 |
| Completeness [%] | 96.0 | 91 |
| Redundancy | 12.8 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 5% PEG3350 100 MM NACITRATE 1MM SPERMINE 5MM DTT, pH 7 |
