2V2Q
IspE in complex with ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-25 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 137.038, 137.038, 137.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.670 - 2.300 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.23400 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.122 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.700 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.580 |
| Number of reflections | 38321 | |
| <I/σ(I)> | 15 | 2.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PROTEIN 30 MG ML-1, RESERVOIR = 1.6 M (NH4)2SO4, 0.1 M NA CACODYLATE, PH 6.5, 0.1 M NABR |
