2V2O
Mutant R59M recombinant horse spleen apoferritin cocrystallized with haemin in basic conditions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-08-16 |
Detector | MARRESEARCH |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 183.801, 183.801, 183.801 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 106.000 - 1.870 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v2n |
RMSD bond length | 0.019 |
RMSD bond angle | 1.621 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.3.0031) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.000 | 2.000 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.070 | 0.500 |
Number of reflections | 22374 | |
<I/σ(I)> | 25 | 3.9 |
Completeness [%] | 99.0 | 95.3 |
Redundancy | 7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.2 | RESERVOIR: CADMIUM SULFATE 0.145M, AMMONIUM SULFATE 0.55M, SODIUM AZIDE 0.003M. DROP: 1UL PROTEIN AND 1UL RESERVOIR, pH 8.2 |