2V2H
The A178L mutation in the C-terminal hinge of the flexible loop-6 of triosephosphate isomerase (TIM) induces a more closed conformation of this hinge region in dimeric and monomeric TIM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-30 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 68.260, 117.990, 81.770 |
Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.180 |
R-factor | 0.1394 |
R-free | 0.18680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ml1 |
RMSD bond length | 0.022 |
RMSD bond angle | 0.041 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.190 |
High resolution limit [Å] | 1.180 | 1.180 |
Rmerge | 0.060 | 0.490 |
Number of reflections | 210261 | |
<I/σ(I)> | 17.01 | 3.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.2 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 0.1 M CITRIC ACID PH 5.5, 20% PEG 6K, 3% TERT.BUTANOL |