2V25
Structure of the Campylobacter jejuni antigen Peb1A, an aspartate and glutamate receptor with bound aspartate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.272, 75.868, 61.708 |
| Unit cell angles | 90.00, 103.31, 90.00 |
Refinement procedure
| Resolution | 60.080 - 1.490 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xt8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.272 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.140 | 0.330 |
| Number of reflections | 75346 | |
| <I/σ(I)> | 13.5 | 2 |
| Completeness [%] | 99.3 | 85.9 |
| Redundancy | 5.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.01 M ZINC CHLORIDE, 0.1 M HEPES PH 7 AND 20 % W/V PEG 6000 |
