2V1C
Crystal structure and mutational study of RecOR provide insight into its role in DNA repair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-26 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.000, 83.200, 66.600 |
| Unit cell angles | 90.00, 106.90, 90.00 |
Refinement procedure
| Resolution | 71.250 - 3.800 |
| R-factor | 0.458 |
| Rwork | 0.459 |
| R-free | 0.44300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1VDD 1w3s |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 4.000 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.140 | 0.640 |
| Number of reflections | 7194 | |
| <I/σ(I)> | 6.5 | 1.4 |
| Completeness [%] | 95.9 | 95.9 |
| Redundancy | 3.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M MES PH 6.5, 10% DIOXANE, 1.6 M AMMONIUM SULPHATE. |
