2V0X
The dimerization domain of LAP2alpha
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.084, 55.683, 122.911 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| Rwork | 0.213 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.379 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.010 | 0.240 |
| Number of reflections | 20002 | |
| <I/σ(I)> | 8.9 | 7.39 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.15 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | VAPOR DIFFUSION AT 4C. PROTEIN CONCENTRATION: 8.5 MG/ML PROTEIN BUFFER: 25MM NAACETATE, PH 5.0 0.5 M NACL 1 MM EDTA 5 MM B-ME PRECIPITANT: 25% (W/V) PEG 3K 0.1 M MES PH 6.5 10 MM TRIS PH 8.5 5 MM B-ME |
