2V0U

n- and c-terminal helices of oat lov2 (404-546) are involved in light-induced signal transduction (cryo dark structure of lov2 (404-546))

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	105
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	35.549, 56.129, 66.778
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.340 - 1.400
R-factor	0.167
Rwork	0.165
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g28
RMSD bond length	0.009
RMSD bond angle	1.293
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.050	0.530
Number of reflections	26704
<I/σ(I)>	36	3.7
Completeness [%]	99.0	100
Redundancy	6.7	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1