2V0O
FCHO2 F-BAR domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-07 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 254.440, 65.680, 89.910 |
| Unit cell angles | 90.00, 110.32, 90.00 |
Refinement procedure
| Resolution | 119.520 - 2.300 |
| R-factor | 0.28 |
| Rwork | 0.278 |
| R-free | 0.31600 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.737 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.940 |
| Number of reflections | 61810 | |
| <I/σ(I)> | 10.2 | 1.6 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 18% PEG4000, 300MM NA ACETATE, 100MM TRIS PH 9 |
