2V0N

ACTIVATED RESPONSE REGULATOR PLED IN COMPLEX WITH C-DIGMP AND GTP- ALPHA-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-14
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	128.965, 132.557, 88.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.710
R-factor	0.216
Rwork	0.215
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w25
RMSD bond length	0.011
RMSD bond angle	1.505
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.100	0.460
Number of reflections	39328
<I/σ(I)>	6.1	6.05
Completeness [%]	95.6	80.2
Redundancy	3.02	2.58

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		PROTEIN SOLUTION: 100 MICROM PLED, 0.2 MM C-DI-GMP, 1 MM GTP-ALPHA-S, 1 MM BECL2, 10 MM NAF, 10 MM MGCL2 RESERVOIR: 0.1 M HEPES, PH 8.0, 0.73 M NA2SO4