2V09
SENS161-164DSSN mutant of Bacillus subtilis Oxalate Decarboxylase OxdC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-23 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | H 3 2 |
Unit cell lengths | 154.602, 154.602, 122.957 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.220 - 1.800 |
R-factor | 0.143 |
Rwork | 0.141 |
R-free | 0.17300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uw8 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.660 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.190 | 1.820 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.090 | 0.310 |
Number of reflections | 49647 | |
<I/σ(I)> | 15.92 | 1.51 |
Completeness [%] | 95.3 | 72.8 |
Redundancy | 6.47 | 2.02 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 8-15% PEG 8000, 0.1 M TRIS PH8.5, 0-15% XYLITOL |