2V07
Structure of the Arabidopsis thaliana cytochrome c6A V52Q variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 57.783, 57.783, 65.145 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.680 - 1.600 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ce0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.343 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.100 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.090 | 0.260 |
| Number of reflections | 16451 | |
| <I/σ(I)> | 19.2 | 5.6 |
| Completeness [%] | 98.9 | 97.5 |
| Redundancy | 7.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 1.26 M AMMONIUM SUPHATE 100 MM AMMONIUM ACETATE PH 4.5 200 MM SODIUM CHLORIDE |
