2UZY

Structure of the human receptor tyrosine kinase Met in complex with the Listeria monocytogenes invasion protein inlb: low resolution, Crystal form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-19
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	139.000, 144.980, 150.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 4.000
Rwork	0.251
R-free	0.30100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1H6T 1shy
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	4.100
High resolution limit [Å]	4.000	4.000
Rmerge	0.220	0.570
Number of reflections	26274
<I/σ(I)>	5.99	2.21
Completeness [%]	99.5	99.9
Redundancy	9.6	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	VAPOR DIFFUSION AT 25 DEGREE C IN SITTING-DROPS. 2 UL PROTEIN (8 MG/ML)PLUS 2 UL RESERVOIR (1.4 M NA/K PHOSPHATE, PH 6.5, 10% PEG 2000 MONO-METHYL-ETHER)