2UZ6

AChBP-targeted a-conotoxin correlates distinct binding orientations with nAChR subtype selectivity.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Collection date	2006-04-23
Spacegroup name	P 1
Unit cell lengths	72.540, 85.754, 121.667
Unit cell angles	90.14, 80.01, 70.64

Refinement procedure

Resolution	67.420 - 2.400
R-factor	0.237
Rwork	0.236
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c9t
RMSD bond length	0.010
RMSD bond angle	1.643
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.420	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.140	0.660
Number of reflections	102052
<I/σ(I)>	4.6	1
Completeness [%]	95.9	95.2
Redundancy	3.82	3.83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1