2UZ0
The Crystal crystal structure of the estA protein, a virulence factor estA protein from Streptococcus pneumonia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.695, 72.128, 101.827 |
| Unit cell angles | 90.00, 94.31, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.307 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.450 |
| Number of reflections | 113097 | |
| <I/σ(I)> | 15.7 | 2.4 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% (V/V) PEG 6000,8% (V/V) ETHYLENE GLYCOL,4% (V/V) 2-METHYL-2,4-PENTANEDIOL,10 MM CYSTEINE,0.1 M TRIS-HCL PH 7.0 |
