2UZ0
The Crystal crystal structure of the estA protein, a virulence factor estA protein from Streptococcus pneumonia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-08 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.695, 72.128, 101.827 |
Unit cell angles | 90.00, 94.31, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.307 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.080 | 0.450 |
Number of reflections | 113097 | |
<I/σ(I)> | 15.7 | 2.4 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 10% (V/V) PEG 6000,8% (V/V) ETHYLENE GLYCOL,4% (V/V) 2-METHYL-2,4-PENTANEDIOL,10 MM CYSTEINE,0.1 M TRIS-HCL PH 7.0 |