2UYU
L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT A88F- E192A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 2 2 |
| Unit cell lengths | 87.097, 87.097, 170.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.960 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ojr |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.710 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.990 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.060 | 0.410 |
| Number of reflections | 47844 | |
| <I/σ(I)> | 16.47 | 3.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.84 | 4.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 40% (V/V) ETHYLENE GLYCOL, 100 MM HEPES (PH 7.5), 5% (W/V) PEG 3000, RESULTING PH: 7.4 |
