2UYT
Structure of L-rhamnulose kinase in complex with ADP and beta-L- rhamnulose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.043, 51.093, 158.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.310 - 1.550 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cgl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.342 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.620 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.050 | 0.370 |
| Number of reflections | 60401 | |
| <I/σ(I)> | 23.2 | 4.7 |
| Completeness [%] | 98.6 | 89.4 |
| Redundancy | 6.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 17% (W/V) PEG 8000, 0.12 M LICL, 20 MM ADP, 20 MM L-RHAMNULOSE-1-PHOSPHATE |
