2UYT
Structure of L-rhamnulose kinase in complex with ADP and beta-L- rhamnulose.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.043, 51.093, 158.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.310 - 1.550 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cgl |
RMSD bond length | 0.010 |
RMSD bond angle | 1.342 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 1.620 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.050 | 0.370 |
Number of reflections | 60401 | |
<I/σ(I)> | 23.2 | 4.7 |
Completeness [%] | 98.6 | 89.4 |
Redundancy | 6.9 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 17% (W/V) PEG 8000, 0.12 M LICL, 20 MM ADP, 20 MM L-RHAMNULOSE-1-PHOSPHATE |