2UYB
S161A mutant of Bacillus subtilis Oxalate Decarboxylase OxdC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-17 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 154.652, 154.652, 121.892 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.180 - 2.100 |
| R-factor | 0.14 |
| Rwork | 0.138 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uw8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.438 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.720 | 2.120 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.140 | 0.380 |
| Number of reflections | 32697 | |
| <I/σ(I)> | 15.28 | 5.39 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.76 | 7.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 8-15% PEG 8000, 0.1 M TRIS PH8.5, 0-15% XYLITOL |
