2UYA
DEL162-163 mutant of Bacillus subtilis Oxalate Decarboxylase OxdC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-23 |
Detector | ADSC CCD |
Spacegroup name | H 3 2 |
Unit cell lengths | 154.857, 154.857, 123.274 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.930 - 2.000 |
R-factor | 0.13 |
Rwork | 0.129 |
R-free | 0.16600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uw8 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.499 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.930 | 2.020 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.100 | 0.290 |
Number of reflections | 38270 | |
<I/σ(I)> | 22.21 | 4.55 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 8.87 | 6.99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 8-15% PEG 8000, 0.1 M TRIS PH8.5, 0-15% XYLITOL |