2UXT
SufI Protein from Escherichia Coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2006-05-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.470, 89.550, 153.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.840 - 1.900 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pf3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.938 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | TNT (5.13.1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.400 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 58949 | |
| <I/σ(I)> | 11.3 | 2.2 |
| Completeness [%] | 98.4 | 98 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2.35 M NACL, 0.1 M IMIDAZOLE PH 8.25 |
