2UXL
X-ray high resolution structure of the photosynthetic reaction center from Rb. sphaeroides at pH 10 in the neutral state, 2nd dataset
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-13 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 139.005, 139.005, 183.554 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 119.520 - 2.880 |
R-factor | 0.178 |
Rwork | 0.176 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pcr |
RMSD bond length | 0.017 |
RMSD bond angle | 2.339 |
Data reduction software | HKL |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.880 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.100 | 0.400 |
Number of reflections | 38279 | |
<I/σ(I)> | 17.2 | 2.3 |
Completeness [%] | 81.3 | 71.4 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 10 | pH 10 |