2UXK
X-ray high resolution structure of the photosynthetic reaction center from Rb. sphaeroides at pH 10 in the charge-separated state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 140.020, 140.020, 235.875 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 119.520 - 2.310 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pcr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.206 |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.220 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.630 |
| Number of reflections | 112221 | |
| <I/σ(I)> | 23.2 | 1.12 |
| Completeness [%] | 93.8 | 60.4 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 | pH 10 |
