2UX9
Crystal structure of the T. thermophilus dodecin R65A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 67.585, 67.585, 169.188 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.880 - 1.400 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mog |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.427 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.880 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.080 | 0.300 |
| Number of reflections | 87239 | |
| <I/σ(I)> | 19.7 | 4.2 |
| Completeness [%] | 98.0 | 96.2 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.4 M AMMONIUM PHOSPHATE |
