2UX8
Crystal Structure of Sphingomonas elodea ATCC 31461 Glucose-1- phosphate uridylyltransferase in Complex with glucose-1-phosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-12 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.97564, 1.13980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.600, 85.700, 151.800 |
| Unit cell angles | 90.00, 105.20, 90.00 |
Refinement procedure
| Resolution | 74.900 - 2.650 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.29500 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.840 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.000 | 2.670 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.100 | 0.650 |
| Number of reflections | 85566 | |
| <I/σ(I)> | 14.1 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1M AMMONIUM ACETATE, 0.1M SODIUM CITRATE PH=4.6, 15% PEGMME, 5MM G1P, pH 4.60 |
