2UX3
X-ray high resolution structure of the photosynthetic reaction center from Rb. sphaeroides at pH 9 in the neutral state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-17 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 139.448, 139.448, 184.577 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 119.520 - 2.500 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pcr |
RMSD bond length | 0.013 |
RMSD bond angle | 2.181 |
Data reduction software | HKL |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.530 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.040 | 0.280 |
Number of reflections | 66474 | |
<I/σ(I)> | 22.89 | 2.65 |
Completeness [%] | 92.1 | 86.3 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | pH 9 |