2UX0
Structure of the oligomerisation domain of calcium-calmodulin dependent protein kinase II gamma
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-21 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 122.954, 158.363, 103.941 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.680 - 2.460 |
R-factor | 0.186 |
Rwork | 0.184 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hkx |
RMSD bond length | 0.012 |
RMSD bond angle | 1.250 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.700 | 2.560 |
High resolution limit [Å] | 2.460 | 2.460 |
Rmerge | 0.060 | 0.460 |
Number of reflections | 29117 | |
<I/σ(I)> | 11.4 | 1.45 |
Completeness [%] | 78.2 | 10.2 |
Redundancy | 2.63 | 0.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.1 M SPG PH 7.0, 60% MPD |