2UWT
X-ray high resolution structure of the photosynthetic reaction center from Rb. sphaeroides at pH 6.5 in the charge-separated state 2nd dataset
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-04-24 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 139.530, 139.530, 184.550 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 121.270 - 2.500 |
R-factor | 0.197 |
Rwork | 0.196 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pcr |
RMSD bond length | 0.016 |
RMSD bond angle | 2.284 |
Data reduction software | HKL |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.530 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.070 | 0.300 |
Number of reflections | 71539 | |
<I/σ(I)> | 21.9 | 3.25 |
Completeness [%] | 98.0 | 70.3 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |