2UWS
X-ray high resolution structure of the photosynthetic reaction center from Rb. sphaeroides at pH 6.5 in the charge-separated state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-04-23 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 139.310, 139.310, 184.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 119.520 - 2.900 |
R-factor | 0.204 |
Rwork | 0.202 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pcr |
RMSD bond length | 0.017 |
RMSD bond angle | 2.416 |
Data reduction software | HKL |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.980 |
High resolution limit [Å] | 2.930 | 2.930 |
Rmerge | 0.090 | 0.480 |
Number of reflections | 60587 | |
<I/σ(I)> | 11.88 | 2.38 |
Completeness [%] | 93.2 | 94.5 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |