2UWI

Structure of CrmE, a poxvirus TNF receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-14
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	41.380, 56.490, 122.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.193 - 2.000
Rwork	0.210
R-free	0.24650
Structure solution method	MAD
Starting model (for MR)	NONE
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	autoSHARP
Refinement software	BUSTER (TNT)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.110	0.880
Number of reflections	20089
<I/σ(I)>	11.1	1.4
Completeness [%]	99.9	100
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	100 NL PROTEIN (6 MG/ML CRME IN 20 MM TRIS 150 MM NACL PH 8.0) AND 100 NL RESERVOIR (0.1 M CITRATE PH 5.0 PLUS 30% W/V PEG 6000) SITTING DROPS AT 20C EQUILIBRATED AGAINST 100 UL RESERVOIRS.