2UV5
Crystal Structure of a CBS domain pair from the regulatory gamma1 subunit of human AMPK in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-03 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.056, 43.885, 55.798 |
| Unit cell angles | 90.00, 118.18, 90.00 |
Refinement procedure
| Resolution | 25.340 - 1.690 |
| R-factor | 0.171 |
| Rwork | 0.168 |
| R-free | 0.22700 |
| Structure solution method | OTHER |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.373 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.2.0019G) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.510 |
| Rmerge | 0.060 |
| Number of reflections | 15986 |
| <I/σ(I)> | 12.5 |
| Completeness [%] | 97.9 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8.00 |
