2UV4
Crystal Structure of a CBS domain pair from the regulatory gamma1 subunit of human AMPK in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-23 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.132, 43.757, 55.654 |
| Unit cell angles | 90.00, 118.83, 90.00 |
Refinement procedure
| Resolution | 34.920 - 1.330 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20700 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.494 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019G) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.920 | 1.380 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 31832 | |
| <I/σ(I)> | 12.5 | 3.1 |
| Completeness [%] | 99.0 | 90.7 |
| Redundancy | 6.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8.00 |
