2UUV
alkyldihydroxyacetonephosphate synthase in P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2006-06-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.800, 98.000, 107.000 |
| Unit cell angles | 114.00, 93.00, 103.00 |
Refinement procedure
| Resolution | 19.980 - 1.990 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.26400 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.683 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 176351 | |
| <I/σ(I)> | 12.4 | 3.4 |
| Completeness [%] | 95.3 | 89 |
| Redundancy | 4.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% PEG4K, 200MM LI2SO4, 100MM TRISCL PH=8.5 |
