2UUI

Crystal structure of Human Leukotriene C4 Synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	F 2 3
Unit cell lengths	169.670, 169.670, 169.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.270 - 2.000
R-factor	0.198
Rwork	0.196
R-free	0.23700
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.017
RMSD bond angle	1.780
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.160	2.110
High resolution limit [Å]	1.700	2.000
Rmerge	0.080	0.820
Number of reflections	44247
<I/σ(I)>	16.1	2.2
Completeness [%]	99.8	100
Redundancy	7.2	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1