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2UUA

Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a Valine-ASL with cmo5U in position 34 bound to an mRNA with a GUC-codon in the A-site and paromomycin.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID14-4
Synchrotron siteESRF
BeamlineID14-4
Temperature [K]90
Detector technologyCCD
Collection date2005-11-28
DetectorMARRESEARCH
Spacegroup nameP 41 21 2
Unit cell lengths401.919, 401.919, 174.592
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution29.880 - 2.900
R-factor0.223
Rwork0.223
R-free0.25400
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1j5e
RMSD bond length0.008
RMSD bond angle1.200
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareCNS
Refinement softwareCNS (1.2)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]100.0003.000
High resolution limit [Å]2.9002.900
Rmerge0.1700.790
Number of reflections308707
<I/σ(I)>8.82
Completeness [%]97.092.3
Redundancy4.24
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
16.5SEE 1J5E, pH 6.5

217705

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