2USH
5'-NUCLEOTIDASE FROM E. COLI
Experimental procedure
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1998-02 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.210, 116.290, 132.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.220 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.25400 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 23.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.270 |
High resolution limit [Å] | 2.220 | 2.220 |
Rmerge | 0.073 * | 0.350 * |
Total number of observations | 196559 * | |
Number of reflections | 70373 | |
<I/σ(I)> | 13 | |
Completeness [%] | 98.4 | 95.1 |
Redundancy | 2.8 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 5 (mM) | |
3 | 1 | drop | 20 (mM) | ||
4 | 1 | drop | 0.05 (mM) | ||
5 | 1 | reservoir | MES | 100 (mM) | |
6 | 1 | reservoir | 15 (mM) | ||
7 | 1 | reservoir | 4 (mM) | ||
8 | 1 | reservoir | mPEG550 | 20 (%(v/v)) |