2UAG
UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM02 |
Synchrotron site | ESRF |
Beamline | BM02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-09 |
Spacegroup name | P 41 |
Unit cell lengths | 65.560, 65.560, 136.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.700 |
Rwork | 0.180 |
R-free | 0.20600 |
Structure solution method | OTHER |
RMSD bond length | 0.009 |
RMSD bond angle | 25.400 * |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.100 | 1.800 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.081 * | 0.294 * |
Number of reflections | 61463 | |
<I/σ(I)> | 36.7 | 9 |
Completeness [%] | 96.0 | 78.8 |
Redundancy | 5.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.5 * | pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 9.8 (mg/ml) | |
10 | 1 | reservoir | 200 (mM) | ||
11 | 1 | reservoir | HEPES | 100 (mM) | |
2 | 1 | 1 | UMA | 1 (mM) | |
3 | 1 | 1 | ADP | 5 (mM) | |
4 | 1 | 1 | 1 (mM) | ||
5 | 1 | 1 | dithiothreitol | 1 (mM) | |
6 | 1 | 1 | HEPES | 20 (mM) | |
7 | 1 | reservoir | monodisperse PEG3350 | 14 (%(w/v)) | |
8 | 1 | reservoir | 50 (mM) | ||
9 | 1 | reservoir | 5 (mM) |