2TMY

CHEY FROM THERMOTOGA MARITIMA (APO-II)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1995-12-15
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 4 21 2
Unit cell lengths	98.657, 98.657, 32.254
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
Rwork	0.181
Structure solution method	SIRAS / MOLECULAR REPLACEMENT
Starting model (for MR)	1tmy
RMSD bond length	0.013
RMSD bond angle	2.190
Data reduction software	SDMS ((DCREDUCE))
Data scaling software	SDMS ((SCALE))
Phasing software	RSS
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.600
High resolution limit [Å]	2.300	2.300
Rmerge	0.067 *	0.184
Number of reflections	6660
<I/σ(I)>	7.8	2.5
Completeness [%]	82.0 *	76
Redundancy	2.9	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.6		0.2M AMMONIUM SULFATE, 0.1M ACETATE BUFFER (PH 4.6), 30% PEG 4000, 15MM MGCL2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	ammonium sulfate	0.2 (M)
3	1	reservoir	acetate	0.1 (M)
4	1	reservoir	PEG4000	30 (%)
5	1	reservoir		15 (mM)