2TLX

THERMOLYSIN (NATIVE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	293
Detector technology	CCD
Collection date	1998-07-01
Spacegroup name	P 61 2 2
Unit cell lengths	94.145, 94.145, 131.616
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.650
Rwork	0.163
R-free	0.18800 *
Structure solution method	ISOMORPHOUS REPLACEMENT
Starting model (for MR)	1lnf
RMSD bond length	0.016
RMSD bond angle	0.800 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.076	0.352
Number of reflections	41876
<I/σ(I)>	6.2	1.8
Completeness [%]	100.0	100
Redundancy	5	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	TLN	4.6 (mM)
2	1	1	DMSO	45 (%)
3	1	1	Tris-HCl	50 (mM)
4	1	1		2.5 (M)