2TDD

STRUCTURES OF THYMIDYLATE SYNTHASE WITH A C-TERMINAL DELETION: ROLE OF THE C-TERMINUS IN ALIGNMENT OF D/UMP AND CH2H4FOLATE

Experimental procedure

Spacegroup name	P 61 2 2
Unit cell lengths	78.400, 78.400, 242.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 2.700
R-factor	0.191 *
Rwork	0.190
RMSD bond length	0.025
RMSD bond angle	3.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.700 *
Rmerge	0.097 *
Number of reflections	10672 *
Completeness [%]	83.0 *
Redundancy	7.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	phosphate
2	1	drop	C-terminal valine	5 (mg/mL)
3	1	drop	ammonium sulfate	1 (%sat)
4	1	reservoir	phosphate	1 (mL)
5	1	reservoir	C-terminal valine	5 (mg/mL)